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 * HERE IS A GOOD LINK FOR REVIEW=:)**

The LE Exam consists of approximately 70-75 questions worth a total of 85 points. The exam is broken down into 4 parts: Part A: General knowledge multiple choice questions (30 points) Part B: A mix of multiple choice and short answer, dealing with the application of knowledge. So far, Part B has always required students to draw a graph. (25 points) Part C: Short answer questions dealing with your ability to apply material learned in the course to real world situations. (15 points) Part D: Multiple choice and short answer, pertaining to the 4 NYS labs performed during the school year. (15 points) The state requires all answers to be recorded in such a way that they cannot be tampered with. As such, __all answers on the test must be written in permanent pen, and mistakes may not be “scribbled out.”__
 * What you absolutely need to know for the Regents Exam! **


 * || ** LE Regents Exam Topics **
 * Topic One: Chemistry of Living Things (p2-4) **


 * Topic Two: The Cell (p5-6) **


 * Topic Three: Nutrition, Photosynthesis and Respiration (p7-9) **


 * Topic Four: Human Body (p10-15) **


 * Topic Five: Reproduction (p16-18) **


 * Topic Six: Genetics (p19-22) **


 * Topic Seven: Evolution (p23-27) **


 * Topic Eight: Ecology (p28-33) **


 * Topic Nine: Experiments and Labs (p34-36) **


 * Topic Ten: State Labs (37-40) ** ||  ||


 * Topic One: Chemistry of Living Things**
 * 1) **I.** [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=295&h=324 width="295" height="324"]]All living things must maintain **homeostasis** in order to stay alive.
 * A)** **Homeostasis**: A balanced state in an organism’s body.
 * B)** Failure to maintain homeostasis results in disease or death.
 * C)** Homeostasis is often maintained using feedback mechanisms.
 * 1) 1. Feedback mechanisms are **cycles** in which the product of one reaction causes another to start or stop.
 * D)** While organisms are balanced, they are not unchanging. The term used to describe the balanced state is **dynamic equilibrium.**
 * 1) 1. **Dynamic Equilibrium:** A balanced state created by many small, opposing changes.

Stores energy in plants (starch)
 * 1) **II.** **Life Processes:** All living things carry out the same basic chemical processes. Taken together, these processes make up an organism’s metabolism.
 * A)** **Metabolism:** All the chemical processes that take place in an organism.
 * 1) 1. **Nutrition:** Using nutrients for growth, synthesis, repair and energy.
 * 2) 2. **Respiration:** Converts **energy** in food into a usable form (**ATP**).
 * 3) 3. **Synthesis**: Making complex chemicals from simple substances.
 * 4) 4. **Transport**: Absorbing and distributing materials throughout the body.
 * 5) 5. **Regulation**: The **control and coordination** of life processes.
 * 6) 6. **Excretion**: Removing of wastes produced by metabolic activities.
 * 7) 7. **Reproduction**: Passes on genes to offspring.
 * 8) **III.** **Inorganic Chemicals**: Simple compounds
 * A)** **Water** ( H2O) : Most common substance in all living things (about 60% of body mass)
 * Needed for chemical reactions ( which won’t happen in “dry” conditions)
 * Dissolves other molecules into **solution,** allowing them to be **transported** through the body.
 * B)** **Oxygen** (O2): Needed by **most** (not all) organisms for **cellular respiration**.
 * Released by plants and algae as a waste product of **photosynthesis.**
 * **Aerobic respiration:** Process that uses oxygen to extract **energy** from glucose (sugar). Used by most organisms.
 * Anaerobic respiration: Process that extracts energy from glucose without using oxygen. Gives less energy, so only used by some simple organisms (some bacteria, yeast). These organisms do not need to breathe in oxygen.
 * C)** **Carbon Dioxide** (CO2):
 * With water, used by plants to make glucose (**photosynthesis)**.
 * Waste product of **aerobic respiration**.
 * D)** **Nitrogen** (N2):
 * Most common gas in air (70%)
 * Needed to make protein.
 * Converted into **nitrates** by soil bacteria. Nitrates are absorbed by plants and then eaten by animals.
 * Excreted as waste in **urine**.
 * E)** **Acids and Bases:**
 * Measured by the **pH** **scale**
 * Very high and very low pHs are usually lethal.
 * pH can affect rates of chemical reactions; for example, digestive **enzymes** work fastest in acidic environments, which is why we make stomach acid (hydrochloric acid, or HCl).
 * 1) **IV.** **Organic Compounds**: Larger, more complex chemicals. Always contain the elements carbon (C)and hydrogen (H). Synthesized from simpler substances (building blocks).
 * A)** [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=352&h=77 width="352" height="77"]]**Carbohydrates:** Sugars and starches
 * 1) 1. Building blocks: Simple sugars
 * 2) 2. Functions:
 * Provides energy
 * || A starch (A) is broken down by an enzyme (B) into two simple sugars (C, D). This is also a good example of the **lock and key model**. ||  ||
 * || A starch (A) is broken down by an enzyme (B) into two simple sugars (C, D). This is also a good example of the **lock and key model**. ||  ||
 * B)** **Lipids:** Fats, oils and waxes
 * 1) 1. Functions:
 * Stores energy (animal fat)
 * Insulation
 * Water proofing
 * Cell membrane
 * C)** **Proteins:** Complex compounds that carry out all the body’s activities.
 * 1) 1. Building blocks: Amino acids
 * 2) 2. Have many different functions as determined by their shape.
 * 3) 3. **Lock and Key Model:** Proteins must have the right shape to “fit” with other molecules.
 * **Changing the shape of a protein will change what it can interact with its function.**
 * 1) 4. Important types of proteins:
 * **Hormones** and **neurotransmitters** – carry messages through the body.
 * Cell receptors – in cell membrane; receive hormones and neurotransmitters.
 * Antibodies – attack foreign **pathogens**
 * **Enzymes**- act as **catalysts**, controlling all chemical reactions in the body.
 * High temperatures will cause enzymes to denature (lose their shape) and stop functioning. This is why high fevers are dangerous.
 * D)** **Nucleic Acids (DNA and RNA)**: Make up genes and chromosomes.
 * 1) 1. **Building blocks**: Nucleotides; molecular bases (ATCGU)

This seems obvious now, but at one time people believed in //spontaneous generation//, the idea that living things regularly emerged from nonliving things. B) **Exceptions to the Cell Theory**
 * Topic Two: The Cell**
 * 1) **I.** **Definition:** The basic unit of structure and function in all living things.
 * 2) **II.** **Cell Theory** has three parts:
 * 3) **1.** **All living things are made of one or more cells.**
 * Unicellular – single celled organisms (amoeba, paramecium)
 * Multicellular – have more than 1 cell; may be only a few cells, or many //trillions// of cells . Almost all structures in multicelled organisms are made of or by cells.
 * 1) **2.** **Cells carry out all life processes.**
 * Everything you do is the result of the work of your cells – walking, talking, even thinking and feeling. When you get sick, it is because your cells are not working correctly.
 * 1) 3. **All cells come from preexisting cells.**
 * 1) 1. **Viruses** are not made of cells. However, they also do not carry out all life processes, so many biologists do not consider them true living things.
 * 2) 2. **The first cell** obviously could not come from another cell.

A) **Atoms** B) **Molecules** C) **Organelles** – Cell structures D) **Cells** E) **Tissues** – Cells with the same structure and function. F) **Organs –** Made of different tissues working together for the same function. G) **Organ Systems -** Groups of organs that work together. H) **Organism**
 * 1) **III.** **Organization**


 * 1) **IV.** **Cell Organelles:** These are the tiny cell parts that make up a cell.
 * 2) **1.** [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=372&h=196 width="372" height="196"]]**Nucleus**
 * Controls the cell
 * Contains hereditary material (chromosomes, genes, DNA)
 * 1) **2.** **Cytoplasm** (technically not an organelle)
 * Fluid/liquid in the cell – mostly water
 * Helps transport material
 * 1) **3.** **Mitochondrion**
 * Carries out **cellular** **respiration**.
 * Gives cell **energy** (Powerhouse of the cell).
 * 1) **4.** **Ribosome**
 * Makes **proteins** from **amino acids**.
 * 1) **5.** **Vacuole**
 * Stores food, water and waste
 * Food vacuoles may digest large molecules.
 * Waste vacuoles may excrete waste out the cell membrane
 * 1) **6.** **Chloroplast**
 * Carries out **photosynthesis**
 * Plant and algae cells only
 * 1) **7.** **Cell Wall**
 * Gives shape, structure and protection.
 * NEVER found in animal cells.
 * 1) **8.** **Cell Membrane**
 * Separates cell interior from environment
 * Controls what enters and leaves the cell using **transport proteins.**
 * Has **receptor molecules** that pick up signals from other cells.
 * Has **antigens** which are protein “tags” that identify the cell (see immune system).

A) **Ingestion:** To take nutrients into the body. B) **Digestion**: To break down nutrients into smaller pieces.
 * Topic Three: Nutrition, Photosynthesis and Respiration**
 * Reminder:** All life processes are **chemical activities** which make up your **metabolism**.
 * 1) I. **Nutrition**: Taking in nutrients (food) for various activities including:
 * 2) 1. growth
 * 3) 2. healing
 * 4) 3. synthesis
 * 5) 4. respiration (energy)
 * 1) 1. Nutrients must be broken down into smaller parts so that they can be absorbed into the blood and cells of organisms.
 * Starches are digested into simple sugars.
 * Proteins are digested into amino acids.

C) **Autotrophic Nutrition:** Organisms take inorganic materials (CO2, H2O) and convert them into organic nutrients (carbohydrates).
 * 1) **1.** Auto = self ; troph = food; so Autotroph = self feeding
 * 2) **2.** **Photosynthesis** is most common form of autotrophic nutrition
 * 3) 3. Plants, algae and blue-green bacteria (cyanobacteria) are common autotrophs.

D) **Heterotrophic Nutrition:** Organisms must consume nutrients from other organisms.
 * 1) 1. Hetero = other so Heterotroph = feeds on others.
 * 2) 2. All animals and fungi are heterotrophs.
 * 3) 3. Includes:
 * **Carnivores**: eats mostly animals
 * **Herbivores**: eats mostly plants or algae
 * **Ominivores**: eats both plants and animals
 * **Decomposers**: breaks down dead matter and waste
 * Decomposers are important for recycling nutrients

A) Requires sunlight, water and CO2. B) Makes glucose (C6H12O6) as food. C) Water and oxygen are waste products. D) Benefits: E) Plant adaptations:
 * 1) II. **Photosynthesis:** Process in which sun’s energy is trapped in the chemical bonds of sugar.
 * 1) 1. Provides food for all plants, animals and other organisms.
 * 2) 2. Provides oxygen to breathe.
 * 3) 3. Removes CO2 from atmosphere.
 * 1) 1. **Chloroplast**: Cell organelle that does photosynthesis
 * 2) 2. Gas exchange:
 * **Stomates :** Pores under a leaf; let gases in and out
 * **Guard cells:** open and close stomates to prevent dehydration
 * 1) 3. Transport:
 * **Xylem and Phloem:** “tubes” transport food and water throughout the plant.
 * ||  || Two different views of the **stomates** and their **guard cells**(X)**.** ||   ||
 * ||  || Two different views of the **stomates** and their **guard cells**(X)**.** ||   ||

A) ATP is the **molecule** all life uses for **energy**. B) Requires **oxygen, glucose and water.** C) **Carbon dioxide** and water are waste products. D) Most organisms carry out **aerobic respiration** (uses oxygen) in their **mitochondria**. E) **Anerobic respiration** does not require oxygen, but gives less ATP (energy) for each molecule of sugar. F) **Photosynthesis and Cellular Respiration are opposite reactions!** They are also important in cycling oxygen, carbon, hydrogen and water through the environment G) **Common mistakes:** //__All__// //organisms, including plants, use respiration to get their energy.// //Breathing is **__not__** respiration. Breathing exchanges the gases needed for respiration. Inhaling and exhaling does not give you ATP.//
 * 1) III. **Cellular Respiration:** Process that takes **energy** from sugar molecules and places it in molecules of **ATP.**
 * No organism can get energy from sunlight or sugar without first putting the energy into ATP.
 * When exercise causes human muscles to run out of oxygen, their cells will do **anaerobic respiration**. The waste product, **lactic acid**, causes muscles to “ burn” so that you will **stop**.
 * “Plants use photosynthesis, animals use respiration.”
 * “Respiration is breathing.”
 * || [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=253&h=99 width="253" height="99" caption="0000576"]] ||
 * || [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=253&h=99 width="253" height="99" caption="0000576"]] ||

“Oxygen is used to breathe.” //This is backwards. Breathing is used to get oxygen which is used for respiration. Without oxygen, you have no respiration, no ATP, and no energy.// //Anaerobic organisms do not need oxygen, and do not have to breathe.//
 * “All living things need oxygen/need to breathe.”

A) All humans (and most other organisms) begin life as a **single** cell. B) Humans grow as a result of **mitosis**(cell division). C) As cells divide, they begin to develop into specialized **tissues**. D) As the body continues to develop, tissues will work together to form **organs**. E) Organs will work together to form **organ systems**. F) Org an systems will work together to help a person **maintain homeostasis**. A) The nervous system **regulates** your body with electrochemical **impulses** B) A nerve cell is also called a **neuron**. C) The main organs of the nervous system are the **brain** and **spinal cord**. D) The **spinal cord** controls reflexes and relays impulses between the brain and body.
 * Topic Four: The Human Body**
 * 1) **I.** **Organization:** The human body is made up of **cells.**
 * 1) 1. This single cell is called a **zygote**.
 * 2) 2. The nucleus of this cell has **all** the genes needed to become a complete organism.
 * 1) 1. This quickly increases the number of cells in the body until there many trillions of cells.
 * 2) 2. Since all new cells come from the same single cell, they all share the same **genes**
 * 1) 1. **Specialization** or **Differentiation:** Process in which a cell changes to have a special shape and function.
 * 2) 2. Cells specialize by turning specific genes on or off.
 * Ex: A white blood cell has turned __off__ all genes needed to make skin, bone, or nerves. It still has those genes, but only the genes needed to be a white blood cell remain turned __on__.
 * 1) **II.** **Nervous System**
 * 1) 1. The chemical portion of a nerve impules is called a **neurotransmitter**.
 * 2) 2. Neurotransmitters released by 1 nerve cell are received by **receptor molecules** in the cell membrane of the next nerve cell.
 * 3) 3. The shape of the receptor molecule determines which neurotransmitter it can receive.
 * || ** Two neurons ** carry an impulse to a muscle cell. (3) shows where a **neurotransmitter** would carry the signal from one cell to the next. ||  ||

A) Uses **hormones** to **regulate** thebody.
 * 1) **III.** **Endocrine System**
 * 1) 1. A hormone is a chemical **message** secreted by endocrine **glands.**
 * 2) 2. Hormones are slower than nerve impulses, but with longer lasting effects.
 * 3) 3. Hormone levels are controlled by **feedback mechanisms**.
 * ||  || ** A feedback mechanism ** ||   ||
 * ||  || ** A feedback mechanism ** ||   ||

B) The **pancreas** makes **insulin** and glucagon which control blood sugar. //Insulin (and glucagon) directly control blood __sugar__ (or glucose) levels, not blood pressure.// C) Adrenal glands make **adrenaline** when the body is under stress. D) **Testosterone** (male), **estrogen** and **progesterone** (female) are the sex hormones. These are made in the gonads (testes for males, ovaries for females).
 * 4.
 * || ** Receptor Molecules ** in the cell membrane can only accept molecules of the correct shape. This is a good example of the **Lock and Key Model.** ||  ||
 * Receptor molecules** on the surface of the cell membrane receive hormones. **As with all proteins, it is the shape of the receptor molecule that determines which hormone it can receive.**
 * **Common mistake:** “Insulin lowers blood pressure.”
 * ||  || ** The brain (nervous system) and some endocrine glands. ** ||   ||
 * ||  || ** The brain (nervous system) and some endocrine glands. ** ||   ||

= IV. Circulatory System = = A) Moves material through the body to the organs and cells that need them. = B) Transported material includes: C) Materials usually enter and leave the blood through **diffusion.**
 * 1) 1. **Nutrients** and **water** from **intestines** to all cells of body.
 * 2) 2. **Oxygen** from lungs to all cells of the body.
 * 3) 3. **Hormones** from glands to **target cells**
 * 4) 4. **Waste** from all cells to the **excretory organs.**
 * 1) 1. **Diffusion**: Process in which material moves from a high concentration to a low concentration.
 * **Ex**: There is a high concentration of oxygen in the lungs, so oxygen will diffuse from the lungs into the blood, which has less oxygen.
 * 1) 2. **Capillaries:** Microscopic blood vessels where diffusion occurs.
 * 2) 1. Hemoglobin: Protein in red blood cells that carries oxygen.

E) [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=237&h=175 width="237" height="175"]] Red blood cells carry oxygen and carbon dioxide
F) **Plasma** is the fluid of the blood. It transports everything //except oxygen//. G) **Platelets** clot the blood.

2. “Oxygen diffuses into and out of the heart.”

 * || ** Red blood cells (A), platelets (B) and white blood cells (C) **

// No materials diffuse in or out of the blood when it is in the heart. This only occurs in capillaries. //
= V. Respiratory System: =

A) Food is broken down so that it is small enough to enter the body tissues/cells. B) The digestive system is a one way passage through the body that includes the **mouth**, **stomach** and **intestines**. C) Food is moved through the digestive system by muscular contractions (peristalsis). D) Undigested food is eliminated as solid waste (**feces**). E) Common mistakes: //The digestive system does not excrete waster (see excretory system)//. //The digestive system gives nutrients. Energy is gained by cellular respiration.//
 * 1) **VI.** **Digestive System:**
 * 1) **1.** Food is broken down mechanically and chemically.
 * 2) **2.** Nutrients and water are absorbed into the body in the small and large intestines.
 * 1) 1. “The digestive system excretes waste.”
 * 1) 2. “The digestive system gives you energy.”
 * || ** Kidneys and Urinary Tract ** - part of the human excretory system. ||  ||

// Feces never enters cells of the body, so technically it is not excreted. The correct term is “eliminated” or “egested.” //
A) The job of the immune system is to protect the body against **pathogens**. B) **Pathogen:** An organism that causes a disease. C) **White Blood Cells** are the main components of the immune system. Destroy pathogen by eating it. D) **Antibodies** are **proteins** made by white blood cells to attack pathogens. E) **Antigens** are protein “tags” that identify a cell or virus. F) **A vaccine is an injection of a dead or weakened pathogen.** G) Antibiotics are drugs used to stop infections by **bacteria**. H) **Common mistake:**
 * 1) **VIII.** [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=325&h=208 width="325" height="208"]]**Immune System**
 * 1) 1. Types of pathogens include viruses, bacteria, and parasites.
 * 1) 1. Different w.b.c’s have different roles, including:
 * Identify pathogens
 * “Tag” pathogens for destruction by other wbc’s.
 * || ** An immune response ** – white blood cells and antibodies attack a virus. ||  ||
 * || ** An immune response ** – white blood cells and antibodies attack a virus. ||  ||
 * Destroy pathogen using chemicals
 * Make **antibodies**
 * 1) 1. Every antibody is specific in its action – it can attack one and only one type of pathogen. As with all proteins, this is because the shape of the antibody must fit its target (lock and key model).
 * 1) 1. Your blood type is determined by your antigens (you can have A or B antigens, both or nether (type O).
 * 2) 2. Any cell of virus with the wrong antigen will be seen as foreign by your immune system, attacked, and destroyed. This is why you must match blood types before receiving blood or an organ transplant.
 * 1) 1. Triggers the body to make antibodies against that pathogen.
 * 2) 2. Effective against both viruses and bacteria.
 * 3) 3. Can only prevent disease, not cure it.
 * 1) 1. Antibiotics will not work against viruses.
 * 2) 2. Unlike vaccines, antibiotics can cure diseases.
 * 1) 1. “Antibodies are cells that attack pathogens.” //Antibodies are proteins, not cells.//

A) The different systems of the body work together to maintain homeostasis. For example: A) Typically the exam asks you to name a disease, what causes it, its effect on the body, and how to prevent/treat/cure it. The most important diseases and disorders for you to know are:
 * 1) **IX.** **Interactions between body systems**
 * 1) 1. //Nutrients from the digestive system are transported to cells by the circulatory system.//
 * 2) 2. //Wastes from the skeletal system are removed by the excretory system.//
 * 3) 3. //The nervous and endocrine systems work together to control the body.//
 * 4) 4. //The immune system protects the nervous system from disease.//
 * 5) **X.** **Diseases and Disorders**
 * 1) **1.** **AIDS**
 * Caused by HIV virus (a pathogen)
 * Weakens human immune system, leaving body vulnerable to other diseases.
 * Spread through bodily fluids, usually sexual contact, intravenous (IV) drug use (sharing needles), or blood transfusions.
 * Can’t be cured, but spread may be prevented by sexual abstinence, “safe” sex (using condoms), not sharing needles, or testing blood before using it for a transfusion.
 * 1) **2.** **Cancer**
 * Caused when a cell reproduces (divides) at an uncontrolled rate, forming a **tumor**.
 * Cancer cells **do not specialize** and take resources from healthy tissue.
 * May be caused by radiation, chemicals (such as asbestos or cigarette smoke), and viruses.
 * Treatments include surgery, radiation therapy, and chemotherapy.
 * 1) **3.** **Diabetes**
 * Affects body’s ability to control blood sugar.
 * Some diabetics may be treated using injections of **insulin** made by genetically engineered bacteria.
 * 1) **4.** **Allergies**
 * Occur when immune system reacts to a harmless substance (such as pollen)
 * **Asthma** is a form of allergy caused by a reaction to dust particles in the air.

A) Advantages: One parent, faster, safer, easier, less energy B) Disadvantage: No variety
 * || ** Mitosis vs Meiosis. ** Notice the number of chromosomes stays the same in mitosis, and is halved in meiosis . ||  ||
 * Topic Five: Reproduction**
 * 1) **I.** **Asexual reproduction:**

A) Advantage: Variety B) Disadvantage: Need to find mate, more risk, more time and energy
 * 1) **II.** **Sexual reproduction:**

A) Used in all forms of asexual reproduction. B) The number and types of chromosomes in the daughter cells are the same as in the parent cell. C) Large organisms use mitosis for growth and healing. D) Simple organisms use it to reproduce. E) One division of a cell à two identical, diploid (2n) cells.
 * 1) **III.** **Mitotic Cell Division (Mitosis)**
 * 1) 1. Diploid: Cell with a two sets of chromosomes in **pairs**.

A) Makes **gametes** used in sexual reproduction. B) One cell divides //twice// à four DIFFERENT haploid (1n) cells. C) Separates pairs of chromosomes so that offspring get **one chromosome of each pai**r from that parent. D) Each daughter cell (gamete) gets only one half of the chromosomes of the “parent” cell.
 * 1) **IV.** **Meiotic Cell Division (Meiosis)**
 * 1) 1. Gamete: Sex cells; egg and sperm
 * 1) 1. Haploid: Cell with **one** set of chromosomes ( ½ normal) and **no pairs**.

A) __ produce and store sperm. B) Testosterone is the male sex hormone, and is made in the testes. C) Penis transfers sperm into the female reproductive system. D) Semen is the fluid that carries sperm.
 * 1) **V.** **Male Reproductive System**
 * 1) 1. Sperm are haploid cells made by __
 * 2) 2. Sperm are produced in large numbers throughout a males life
 * 3) 3. Sperm are smaller than the egg and mobile
 * 4) 4. Sperm only provide offspring with 23 chromosomes – everything else is in the egg.
 * 1) 1. Semen contains sugar to give sperm energy.

A) produce eggs. B) The menstrual cycle lasts 28 days (on average) C) The ___ carries the egg to the uterus. D) The__ is the womb where the baby will develop. E) The vagina is the birth canal where the baby will leave the body.
 * 1) **VI.** **Female Reproductive System**
 * 1) 1. Eggs are haploid cells made by ___.
 * 2) 2. Females are born with all eggs they will ever need.
 * An egg is not fully developed until ovulation
 * Females are born with millions of eggs, enough for several lifetimes.
 * 1) 3. Eggs are largest cells in the body.
 * 2) 4. Eggs do not move on their own.
 * 3) 5. Contain 23 chromosomes and all cell parts (mitochondria, ribosomes,etc) that the offspring will need to grow and develop.
 * 1) 1. Ovulation – release of an egg (typically 1 per cycle)
 * 2) 2. Menstruation – shedding of the uterine wall if fertilization doesn’t occur
 * 3) 3. If pregnancy occurs, the menstrual cycle will temporarily stop.

B) A zygote develops in the following order: C) The **placenta** transfers nutrients and oxygen from the mother’s blood into the blood of the fetus through the process of **diffusion**. D) The child is vulnerable to alcohol, drugs, etc because organs and systems are still developing.
 * 1) **VII.** **Development**
 * A)**
 * || ** Fertilization ** restores the correct number of chromosomes . ||  ||
 * Fertilization occurs in the fallopian tube.**
 * 1) 1. [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=225&h=98 width="225" height="98" caption="0000461"]]A fertilized egg is called a **zygote**.
 * 2) 2. Fertilization restores the complete set of chromosomes, so the zygote is diploid (23 from the egg + 23 from the sperm = 46).
 * 1) 1. Cleavage – A form of **mitosis** - cells divide but do not differentiate
 * 2) 2. Differentiation – Cells form into tissues and organs
 * 3) 3. **Implantation** in the walls of the **uterus**.
 * 4) 4. **Fetus** - most major organs are formed (but not completed)
 * Continues to grow through cell division (mitosis)
 * 1) 1. The blood of the mother and fetus do not mix.
 * 2) 2. The fetus is attached to the placenta by the **umbilical cord**.
 * 3) 3. Waste produced by the fetus is also removed by the placenta.
 * Waste (CO2, urea, salts) //diffuse// from placenta into mother’s blood.
 * Since the fetus does not eat solid food, it does not have to eliminate feces.


 * ||  || ** Early development – ** Fertilization (A) forms a single celled **zygote** which then begins the process of **cleavage (B)** which will eventually create a layered ball of cells that will form the embryo. ||   ||
 * ||^  ||   ||||   || ** Late Development ** – The fetus pictured here is nearly ready to be born. Note the umbilical cord, placenta and amniotic sac. ||   ||
 * ||||||  ||||   || ** Late Development ** – The fetus pictured here is nearly ready to be born. Note the umbilical cord, placenta and amniotic sac. ||   ||
 * ||||||  ||||   || ** Late Development ** – The fetus pictured here is nearly ready to be born. Note the umbilical cord, placenta and amniotic sac. ||   ||
 * ||||||  ||||   || ** Late Development ** – The fetus pictured here is nearly ready to be born. Note the umbilical cord, placenta and amniotic sac. ||   ||
 * ||||||  ||||   || ** Late Development ** – The fetus pictured here is nearly ready to be born. Note the umbilical cord, placenta and amniotic sac. ||   ||

A) Humans have **46** chromosomes, or **23** homologous pairs. B) **Chromosome pairs** carry genes for the same traits. C) **Sex chromosomes –** In humans, females are XX and males are XY D) **Common mistake**:“Humans have 23 chromosomes (or 46 pairs of chromosomes, or some other incorrect number).” //These numbers are often confused. You must memorize them correctly.// A) Each chromosome has hundreds or thousands of genes. B) **Each gene codes for a particular protein**. //Genes carry the instructions to make protein. The genes themselves are made from nucleic acids.//
 * Topic Six: Genetics**
 * 1) **I.** **Chromosomes:**
 * 1) 1. **Homologous:** chromosomes with the same size, shape and genes.
 * 1) 1. Most organisms have two genes for each trait - 1 from each parent, 1 on each member of the homologous pair.
 * 1) 1. The Y chromosome is much smaller than the X, so it is missing many genes. This means many genes on the X chromosome do not have a “partner” so:
 * If a male has a recessive trait on the X chromosome, the Y chromosome will not be able to “hide” it with a dominant gene, so...
 * This makes males more likely to have some traits (like color blindness). These are called **sex linked traits.**
 * 1) **II.** **Chromosomes and Genes**
 * 1) 1. **Common mistake:** “Genes/DNA are made from protein.”
 * 1) 2. While genes determine our traits, **the environment can affect expression of genes.**

A) DNA is the chemical that makes up your genes and chromosomes. B) The shape of a DNA molecule is a **double helix**, which resembles a twisted ladder. C) The shape of DNA allows it to **replicate** (copy) itself almost perfectly. D) DNA is made of 4 bases: **A, T, C and G** A) A **codon** is a sequence of **three bases** in DNA**.** B) **RNA** carries the genetic code to **ribosomes**.
 * 1) **III.** [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=307&h=188 width="307" height="188" caption="http://www.pinkmonkey.com/studyguides/subjects/biology-edited/chap8/fig_7.gif"]]**DNA**
 * 1) 1. Analogy: If your genes and chromosomes are the “instruction manual” for your body, DNA would be the paper it is printed on.
 * 1) 1. Base pairs: A - T and C- G
 * in RNA the pairs are A - U and C- G
 * 1) **IV.** **Protein Synthesis**: This is how genes control your body:
 * Each codon represents a specific **amino acid**.
 * Ribosomes assemble amino acids in the same order that they are listed in the DNA codons.
 * The amino acids will make a **protein**.
 * The order of the amino acids (determined by the DNA sequence) determines the **shape** of the protein.
 * The shape of a protein determines its **function**.
 * Therefore: The sequence of bases in DNA will determine the functions of all the proteins in the body.
 * The proteins build and run the body.

A) Can only be passed on if they occur in reproductive cells (sperm or egg). B) Common **mutagenic agents** include **radiation, chemicals and viruses**. C) **Gene mutations** may cause a change in a gene which can change the shape of a protein. This will have an effect on the way the protein works (if it still works at all). D) **Chromosome mutations** are usually caused when a person inherits too many or too few chromosomes. A) **Selective breeding**: Breeding organisms to produce offspring with desired traits. B) **Genetic engineering:** Inserts a gene from one organism into the DNA of a different organism. C) New technologies (**karyotyping, DNA fingerprinting**) are making it easier to diagnose and treat genetic disease, though we cannot yet cure them. D) Genetic research has posed many **ethical** problems (ie right and wrong) that science alone cannot answer.
 * 1) **V.** **Mutations: Any change in the genetic material of an organism.**
 * 1) 1. Mutagenic agent: Any environmental factor that causes a mutation.
 * 1) 1. Gene mutations are caused when DNA bases are in some way changed.
 * 1) 1. Chromosome mutations affect many genes at once. Most are lethal
 * 2) 2. **Down’s Syndrome**: Non lethal mutation, caused by inheritance of an extra copy of chromosome 21. (Note – only chromosome 21 can cause Down Syndrome).
 * 3) **VI.** **Genetic technology:**
 * 1) 1. **Inbreeding** – Breeds organisms with the same traits ( ex: beagle x beagle)
 * 2) 2. **Outbreeding** – Breeds organisms with different traits ( ex: lion x tiger)
 * 3) 3. Cannot be used to breed completely unrelated species.
 * 1) 1. **Restriction enzymes** are used to cut the DNA segments.
 * 2) 2. Organism that receives the new gene will begin to make the protein coded for by that gene.
 * 3) 3. [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=314&h=154 width="314" height="154"]]The new protein/enzyme/hormone will be exactly the same as the one produced by the original organism.
 * 4) 4. Bacteria are often used because they are cheap, simple, and reproduce quickly.
 * 5) 5. The example of gene splicing you MUST know:
 * **Bacteria have been engineered to make insulin for diabetics.**
 * **Bacteria have been engineered to make human growth hormone.**
 * In both cases the engineered hormones are safe to use because they are identical to normal human hormones.
 * 1) 1. **Karyotype:** A photograph of an organism’s chromosomes.
 * Can determine if a person has a chromosome disorder such as Down Syndrome.
 * 1) 2. **DNA finerprinting**, or gel electrophoresis, creates banded patterns based on a person’s DNA base sequence.
 * Each fingerprint is unique, so it can be used to identify people.
 * Fingerprints of relatives are similar to each other, so can be used to determine genetic relationships between two people, or even two groups of organisms.
 * 1) 1. [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=301&h=237 width="301" height="237" caption="0000526"]]Ethics: Study of what is morally right or wrong.
 * ||  || ** A karyotype ** shows all 23 pairs of human chromosomes. Note the last pair identifies this as a male. ||   ||
 * ||  || ** A karyotype ** shows all 23 pairs of human chromosomes. Note the last pair identifies this as a male. ||   ||

A) **Charles Darwin:** B) The modern theory (which combines Darwin’s ideas with genetics and other new ideas) contains the following ideas: A) Changes need to be long term – species do not evolve because of changes in the season. B) Changes can include: C) Environmental change **DOES NOT** **CAUSE** evolution to occur. A temperature or climate change does not itself force a species to change its inherited characteristics. A) **Variation:** Members of a species are different from each other due to mutations and sexual reproduction. B) **Overproduction**: Too many offspring are produced. Most will die before reaching maturity. C) **Struggle for Existence/Competition**: Offspring must compete with each other to survive and reproduce. D) **Survival of the Fittest:** E) **Differential Reproduction**: F) **Repeat**: Evolution does not happen overnight. It takes many generations of repetitive selection to weed out the unfit traits and preserve the “better” traits.
 * Topic Seven: Evolution**
 * 1) **I.** **Evolution**: A progressive change in a population over time.
 * 2) **II.** **Modern Theory of Evolution:**
 * 1) 1. Was not the first to think of evolution, but he did figure out how it works (mostly).
 * 2) 2. Darwin didn’t know about genes, so he couldn’t know about mutations.
 * 1) 1. Earth is old (4.55 billion years) and is constantly changing.
 * 2) 2. As the environment changes, evolution causes species to adapt to their environment.
 * 3) 3. **Natural selection** is the **mechanism** that causes species to change.
 * 4) 4. **Common Descent:** Modern species evolved from earlier, different species and share a **common ancestor**.
 * 5) 5. Species that can not adapt become **extinct**.
 * 6) 6. New traits arise in a species from **mutations** and the **recombination of genes** in sexual reproduction.
 * 7) **III.** **Environment and Evolution**: Species usually evolve when the environment changes.
 * 1) 1. Climate change
 * 2) 2. Change in temp
 * 3) 3. Change in water availability
 * 4) 4. Change in food availability
 * 5) 5. Introduction of new species (new food, new predator)
 * 6) 6. Species may be moved to a new location (accidentally taken to an island for example)
 * 1) 1. If this were the case, then all species would be able to adapt to the new environment, and extinction would be a very rare event.
 * 2) **IV.** **Natural Selection:** The basic steps in natural selection are:
 * 1) 1. No variation = no evolution or natural selection, as there is nothing to “select.”
 * Species with no variation are usually the first to die when the environment changes.
 * 1) 1. Offspring who inherited “fit” traits are, on average, better able to get resources, escape from predators and find mates.
 * 2) 2. Offspring with “unfit” traits will have more difficulty surviving and finding mates.
 * 3) 3. **Fitness:** A measure of how well a trait helps an organism to survive and reproduce in its environment. Note that there is no absolute rule for fitness – what is fit in one environment may be unfit in another.
 * 4) 4. **Note:** This “selection” is not a conscious act – no one is “choosing” who survives and who doesn’t. It is the result of the conditions of the organism’s environment.
 * 1) 1. More fit organisms reproduce and pass on their genes than unfit organisms.
 * 2) 2. On average, the next generation will have more traits from the “fit” parents than the unfit ones.
 * 3) 3. NOTE: Traits are still inherited randomly. Individuals offspring of “fit” parents can still inherit “unfit” traits (though it will be unlikely to survive and reproduce). It is only by looking at the ENTIRE population that you will see the “fit” traits become more common.

A) **Geographic Isolation**: A population is separated into 2 or more different habitats. B) **New variation and adaptation:** Each population adapts to its new environment in different ways. This results in physical and genetic differences between the two populations. C) **Add time:** The longer two populations are apart, the greater their differences will become. D) **Reproductive Isolation:** Eventually the populations change so much that they are unable to interbreed, even when brought together. A) **__Kingdoms__** are large groups of related organisms (fungi, bacteria, protists, animals, plants). B) A **__species__** is able to successfully reproduce amongst its members. C) Branching tree diagrams (cladograms) are often used to show evolutionary relationships. A) **Fossil** **record** preserves extinct speices as well as transitional forms between different types of organisms. B) **Radiometric Dating** of rocks consistently confirm the age of the Earth and fossils C) Comparisons of the anatomy (physical structures), embryology (development), chemistry and genes of species confirm expected relationships. D) **Direct observation**: Humans have seen evolution occur both in nature and in the lab. Examples include: A) “Stronger organisms are more fit than weak ones.” //Evolutionary fitness is not physical fitness. Fitness is determined by who is better adapted to survive in a particular environment and who can pass on their genes. Stronger is not always better. There are many examples of species for whom it is better to be slow, weak, or stupid, than fast, strong or smart. It all depends on the environment you are in.// B) “The organism evolved to live in its environment.” //Individual organisms do not evolve. Only populations can evolve.// C) “The organism could not adapt and it went extinct.” //Individual organisms die; they cannot go extinct. Only species can become extinct.// D) “The bacteria became resistant to antibiotics when they were exposed to them” //To evolve, variations must exist in a species BEFORE the environment changes (pre-adaptation). Bacteria who did not already have a resistance to antibiotics would die when exposed to them, a Chihuahua who is left out in the cold will not grow long, warm fur and a squirrel who plays in traffic will not evolve automobile resistance.// E) “Giraffes got long necks because they __needed__ them to eat leaves at the tops of trees.” //Species do not evolve traits because they need them - Life would be much better if we could! Short necked giraffes were never given long necks any more than slower antelopes are given speed when confronted by a predator. The reason there are no short necked giraffes (or slow antelope) is that they were out competed by members of their species with more fit traits. Better answers are//
 * 1) **V.** **Speciation:** The process of making a new species from an existing one.
 * 1) 1. **Once two populations can no longer breed together, they are considered new species.**
 * 2) **VI.** **Classification**- Organisms are classified based on their evolutionary relationship.
 * 1) 1. Note that this is not a perfect definition – Lions and tigers can breed together, as can dogs and wolves. Because evolution is a constantly ongoing and gradual process, there are many, many examples in which the lines between species are blurry (see Ring Species)
 * 1) **VII.** **Evidence:** Evidence in support of evolution comes from many fields:
 * 1) 1. **Bacteria** evolving resistance to antibiotics.
 * 2) 2. **Insects** evolving resistance to pesticides.
 * 3) 3. Modeling natural selection with selective breeding to alter a species’ traits.
 * 4) 4. Observed examples of speciation
 * 5) **VIII.** **Common Mistakes**
 * “Giraffes evolved long necks because the ones with longer necks were better adapted to get food than short neck giraffes.”
 * “Giraffes evolved long necks because more short necked giraffes died, and more long neck giraffes lived and reproduced.”


 * ||||||||||  ||||   || Deeper fossils are typically older than those above them. ||   ||
 * ||  ||||   || ** Evolutionary trees ** can show the relationship between living and extinct species. ||   ||
 * ||||||||  ||||   || ** Transitional forms ** for many species can be found in the fossil record. This diagram shows the evolution of the modern horse from a small, many-toed ancestor. ||   ||
 * ||||  || ** Homologous Structures ** reveal that the same body parts can be modified to perform different functions. ||   ||||^   ||
 * ||||||||  ||||   || ** Transitional forms ** for many species can be found in the fossil record. This diagram shows the evolution of the modern horse from a small, many-toed ancestor. ||   ||
 * ||||  || ** Homologous Structures ** reveal that the same body parts can be modified to perform different functions. ||   ||||^   ||
 * ||||  || ** Homologous Structures ** reveal that the same body parts can be modified to perform different functions. ||   ||||^   ||

A) **Habitat:** Where an organism lives. B) **Niche:** What an organism does, primarily determined by when, where, and how it obtains food. C) **How organisms interact with each other:** Ex: A squirrel and a chipmunk compete for acorns. A) **Abiotic** Factors: Non-living B) **Biotic** Factors: Living C) Levels of organization:
 * Topic Eight: Ecology**
 * 1) **I.** **Ecology:** The study of the interaction between organisms and their environment.
 * 1) 1. **Two species in an ecosystem trying to fill the same niche will create competition**, which usually results in only one species occupying a niche at any one time. Organisms with similar needs will often divide resources to reduce competition (ex: birds eat insects during the day, bats eat them at night).
 * 1) 1. **Competition**: Two organisms need the same resource at the same time.
 * 1) 2. Feeding: One organism feeds on another.
 * **Producer** – An **autotroph**; organisms that makes its own nutrients from simple substances.
 * **Consumer** – A **heterotroph**; may be an herbivore, carnivore, omnivore or decomposer.
 * 1) 3. **Symbiosis**: A close relationship between two organisms in which at least one benefits.
 * Can include 2 organisms working together for mutual benefit (bee and flower) or 1 organism harming another (parasite-host).
 * 1) **II.** Organization
 * 1) 1. **Population:** All members of __one__ species in an area.
 * 2) 2. **Community:** All species in an area.
 * 3) 3. **Ecosystem:** All species in an area __and__ their abiotic factors.
 * 4) 4. **Biosphere:** Portion of Earth where life is found.

A) **Carrying capacity**: The largest population an ecosystem can support. B) **Limiting factors**: Anything which limits the size of a population, including food, water, predators, disease, competition, territory and climate. C) **Overpopulation**: When a population exceeds the carrying capacity. Usually results in a large number of organisms dying off until a new balance is reached. C) **Food chain –** Shows 1 way that energy can “flow” through an ecosystem. D) **Food web** – Shows many energy pathways. E) **Energy pyramid**: Shows that energy gets lost with each step in a food chain
 * 1) **III.** **Populations**: A given area can only supply enough resources for a limited number of organisms.
 * 1) **IV.** **Energy in an Ecosystem**
 * A)** **The sun provides all energy for life on Earth.**
 * B)** **Sun’s energy is stored in the chemical bonds of food through the process of photosynthesis.**
 * 1) 1. Energy is lost because every organism uses some of the energy for it’s own life processes.
 * 2) 2. **Only about 10% of energy is passed from one step to the next**. This is why populations of predators are typically less than the populations of their prey.

A) **Diverse ecosystems** (those with many types of species) are more stable than ones that are not diverse. B) As habitats are lost and species become extinct, biodiversity is reduced. This is considered to be bad because: A) The organisms in each stage of succession change the environment, and allow new organisms to move in and replace them. B) **Climax Community**: The final stage of succession. C) Any temporary disruption of a community will begin the process of succession all over again. A) **The primary reason humans have a negative impact on the environment is because the human population is growing, which places a greater demand on resources such as food, water and space.** B) **There are no easy solutions to any ecological problem. Every solution can have negative consequences. Choosing the “right” actions requires weighing the benefits with the risks.** C) Human actions that generally have a negative impact on the environment include: D) **Actions being taken by humans to reduce or repair damage to the environment include:**
 * 1) **V.** **Biodiversity** refers to the variety of life on earth.
 * 1) 1. **Ecosystems with low diversity are less stable than ecosystems with more diversity,**
 * 2) 2. **Ecosystems with low diversity take longer to recover from environmental changes**
 * 3) 3. **Humans use organisms for many things such as food and medicine; by reducing biodiversity we are losing potentially valuable resources.**
 * 4) **VI.** **Ecological Succession**: Process in which one community is gradually replaced by another, until a **climax community** is established.
 * 1) 1. The climax community is determined by the local climate.
 * [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=693&h=380 width="693" height="380" caption="0000316"]]Ex: Kansas has very fertile soil, but not enough rain to support trees, so succession stops with grasses and shrubs.
 * Ex: If a forest fire kills all the trees in an area, succession will eventually return the area back into a forest, but it much first pass through all the necessary stages.
 * 1) **VII.** **Human Impact**: Human actions can have both a negative or positive impact on the environment.
 * 1) 1. Development/industrialization
 * 2) 2. Pollution
 * 3) 3. Farming
 * 4) 4. Overhunting,/overgrazing
 * 5) 5. Clear cutting/deforestation
 * 6) 6. Introduction of foreign species
 * 1) 1. Recycling wastes
 * 2) 2. Conserving available resources
 * 3) 3. Using cleaner resources (ex: solar over fossil fuels)
 * 4) 4. Protection of habitats and endangered species
 * 5) 5. Use of biological controls instead of pesticides and herbicides
 * 6) 6. Farming native plants (ex: cocoa in the rainforest)
 * 7) 7. Planting trees to replace those cut down.
 * 8) 8. Rotating crops or planting cover crops to reduce soil loss.
 * 9) 9. Passing laws to control pollution, land management, hunting, fishing, etc.

A) Industrialization B) Acid rain C) Depletion of ozone layer – **unrelated to global warming!** D) Loss of habitat (ex: deforestation) E) Loss of diversity F) Global warming G) Introduced species
 * 1) **VIII.** **Specific Environmental Problems:**
 * 1) 1. Cause: Development of factories, cities, etc
 * 2) 2. Negative effect: Increases use of resources, increases human population, increases pollution, acid rain, global warming, habitat loss
 * 3) 3. What can be done: Plan carefully, use cleaner technology, pass laws to regulate development
 * 1) 1. Cause: Burning fossil fuels, releasing SO2 and NO2 in the air, which react with rain water to form acid.
 * 2) 2. Negative effect: Acidification of lakes and soil, damages wildlife and plants, increases erosion of buildings
 * 3) 3. What can be done: Use buffers to neutralize acids in lakes and rivers, reduce use of fossil fuels
 * 1) 1. Cause: Use of CFC’s (chlorofluorocarbons) in aerosol sprays and coolants
 * 2) 2. Negative effect: Increases skin cancer rates
 * 3) 3. What can be done: Don’t use CFCs – use safer chemicals.
 * 1) 1. Cause: Industrialization, farming, increasing human population
 * 2) 2. Negative effect: Loss of **biodiversity**
 * 3) 3. What can be done: Pass laws to protect natural areas (national parks, wildlife sanctuaries), control population growth
 * 1) 1. Cause: habitat loss, over hunting/fishing/harvesting, pollution, introduced species
 * 2) 2. Negative effect: ecosystems are less stable, humans lose potential resources
 * 3) 3. What can be done: pass laws to protect species, regulate hunting
 * 1) 1. Cause: increased emissions of greenhouse gases from fossil fuels (especially CO2)
 * 2) 2. Negative effect: Climate change can lead to loss of habitat and species
 * 3) 3. What can be done: Cleaner technologies, alternative energy, reduce CO2 emissions.
 * 1) 1. Cause: humans moving around planet (tourism, business, etc)
 * 2) 2. Negative effect: new species may outcompete native wild life
 * 3) 3. What can be done: control importation of new species, use **biological controls** (natural predators and disease) to control population


 * || ** Example of a Controlled Experiment: **
 * Hypothesis: **

If people chew gum it will improve their memory. Chewing gum – some people will chew gum, some will not.
 * Independent variable: **

Memory – all groups should have their memory checked both before and after the experiment to see if it was improved.
 * Dependent variable **

Group that chews gum.
 * Experimental Group **

Doesn’t chew gum (remember – the control group never receives the new treatment)
 * Control Group **


 * Constants **

Should be the same for both groups: People in each group should be of similar health with similar memory, with similar mixes of sexes, ages, and ethnicities. Each group should also be tested in the same way.

You should test people’s memories both before and after the experiment. ||  || A) **Observation:** What is seen or measured. B) **Inference:** A conclusion based on observation or evidence. C) **Hypothesis:** A __prediction__ based on available evidence. A good hypothesis states both cause and effect. D) **Theory:** An __explanation__ of natural events that is supported by strong evidence. //This is an incorrect use of the word “theory” in a scientific context. A scientific theory is **__not__** a simple guess or conjecture, and **__is__** strongly supported by evidence.//
 * Data Collected **
 * Topic Nine: Experiments and Labs**
 * 1) **I.** **Terms:**
 * 1) 1. A correct hypothesis can be **tested** and **falsified** (proven incorrect) using an **experiment**.
 * 2) 2. The easiest way to write a correct hypothesis is as an **“if-then”** statement. (ex: __If__ I give patients this pill, then they will not get sick.)
 * 1) 1. Theories tie together many scientific facts, hypotheses and laws.
 * 2) 2. **Common Mistake:** “Theories are things that are opinions, or are not proven.”

A) **Experimental group:** Group being tested or receiving treatment. B) **Control group:** “Normal” group. Should be identical to experimental group in every way except //one//: it does not receive the new treatment.
 * 1) **II.** **Controlled Experiments:** Compares the results of an experiment between one or more experimental groups with a “normal” group.

C) **Placebo:** A sugar pill or other “fake” treatment given to the control group. Usually only needed when using human subjects.

D) **Independent Variable:** Variable that is being tested (ex: new drug, new fertilizer).
 * 1) 1. The “If” part of an “If-then” hypothesis.
 * 2) 2. The independent variable is always plotted on the X axis.

E) **Dependent Variable:** Variable that is measured at the end of an experiment; the results.
 * 1) 1. The “then” part of an “If-then” hypothesis.
 * 2) 2. The dependent variable is always plotted on the Y axis.

A) **Data tables** are used to organize data which will be plotted in a graph. order.
 * 1) **III.** **Graphs and Data Tables**
 * 1) 1. First column in the table is for the **independent variable**.
 * 2) 2. Second column is another for the **dependent variable.**
 * 3) 3. Each column should be titled, and include units of measurement.
 * 4) 4. Data in the table must be arranged in ascending or descending

B) Both the x and y axis of the graph must be labeled or titled. These labels are typically the same ones used in the data table. Once again units of measurement must be written with the title.
 * 1) 1. The **independent variable** is always plotted on the **x-axis**.
 * 2) 2. The **dependent variable** is always plotted on the **y-axis**.

C) The x and y axis must be numbered.
 * 1) 1. **These numbers must increase by a uniform increment** (that is you must count by 1’s, 2’s, 5’s, 10’s, etc).
 * 2) 2. **Your numerical scales should take up most of the axes**. Squeezing it all into the bottom corner makes the graph impossible to read and no credit will be given.
 * 3) 3. The **numbers must line up with the grid lines** of the graph, not with spaces between them.
 * 4) 4. **You do not need to start numbering your axis with 0**.

D) To date, all graphs drawn on the LE Regents have been **line graphs**. Any student who draws a bar graph instead of a line graph will be denied credit for this part of the test.

E) All points plotted on your graph must be **surrounded by a circle** (or sometimes a square or triangle, depending on the directions).

A) **Can be repeated the same way and get the same results.**
 * 1) **IV.** **Characteristics of a good experiment:**

B) **Have large sample size/many test subjects.**

C) **Are performed over longer periods of time.**

D) **Test only one independent variable.** All other characteristics of the tested groups should be the same.

E) **Are peer reviewed –** examined by other scientists to determine its accuracy.

F) **Must test the hypothesis and show whether it is wrong or right**.

G) **Is objective –** the experiment and conclusion are fair and **unbiased**. Fact and opinion are not mixed.

H) The experiment follows established **ethical** and **legal** standards.

A) **Part A** B) **Part A2** C) **Part B**
 * Topic Ten: The State Labs (Part D)**
 * 1) **I.** **Making Connections (aka The Clothespin Lab)**
 * 1) **1.** **What you did:** measured how exercise affected pulse rate.
 * 2) **2.** **What you learned:** exercise increases pulse rate
 * 1) **1.** **What you did:** Squeezed a clothespin for 1 minute, then squeezed it again for another minute
 * 2) **2.** **What you learned:**
 * If you squeezed more the second round, it may have been because your finger muscles were “warmed up” from increased circulation.
 * If you squeezed less the second round, it may have been because your finger muscles were fatigued.
 * 1) **1.** **What you did:** Designed an experiment to test how exercise affects squeezing a clothespin.
 * 2) **2.** **What you learned:** How to design an experiment (see pages 34-36).

A) **What you did:** Compared 4 species of plants, based on structural (physical) and molecular (chemical and genetic) traits. B) **What you learned:** An **electric current** carries the DNA fragments through the gel, separating them according to size (smaller pieces of DNA are carried farther from the well than larger pieces). **This creates a pattern of bands which is unique for every organism.** Related organisms will show similar banding patterns because their DNA have similar base sequences. ||  ||
 * 1) **II.** **Relationships and Biodiversity (//Botana curus// lab)**
 * 1) **1.** Species that are related share similar traits.
 * 2) **2.** Different techniques (such as **gel electrophoresis** and **paper chromatography**) can be used to determine relationships between organisms.
 * 3) **3.** Endangered species should be protected because they may offer benefits to humans.
 * ||  || **Gel Electrophoresis –** A technique used to show how species are related to one another.
 * Restriction enzymes** cut DNA into fragments, which are placed into a well in a gel plate.
 * Restriction enzymes** cut DNA into fragments, which are placed into a well in a gel plate.

A) **What you did:** Played different finch species competing for food. B) **What you learned:** Different environmental conditions (food) favored different species of finch, allowing some to survive and reproduce, but not others.
 * 1) **III.** [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=261&h=216 width="261" height="216" caption="0001426"]]**Beaks of Finches**



A) **Part A**
 * 1) **IV.** **Diffusion Through A Membrane**
 * 1) **1.** **What you did:**
 * Made a model cell using **dialysis tubing**.
 * Put glucose and starch inside your “cell.”
 * Put starch indicator (iodine) outside cell
 * 1) **2.** **What you saw:**
 * Inside of cell turned black because iodine diffused //into// the cell
 * Because outside of the cell was not black, you know the starch did not diffuse through the membrane.
 * Used blue glucose indicator (Benedict’s solution) to see that glucose did diffuse through the membrane.
 * 1) **3.** **What you learned**
 * Small molecules (glucose, iodine) can **diffuse** through a membrane on their own.
 * Large molecule (starch) cannot diffuse through a membrane on their own.
 * You can use indicators to identify the presence of specific substances.

B) **Part B**
 * 1) **1.** **What you did:**
 * Looked at red onion cells under the microscope.
 * [[image:http://elazzari.wikispaces.com/site/embedthumbnail/placeholder?w=253&h=157 width="253" height="157" caption="0000402"]]Added salt water to the onion cells.
 * Added distilled (pure) water to the onion cells.
 * 1) **2.** **What you saw:**
 * Salt water caused the onion cells to shrivel.
 * Distilled water cause the cells to swell back to normal.
 * 1) **3.** **What you learned:**
 * **Salt water causes water to diffuse out of a cell.**
 * **In pure water, water will diffuse into a cell.**